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AFRH CONFERENCE 2019 ABSTRACTS
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AFRH Conference 2019 Abstracts


Date of Web Publication03-Aug-2021

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Source of Support: None, Conflict of Interest: None

DOI: 10.4103/2468-8452.322999



How to cite this URL:
. AFRH Conference 2019 Abstracts. Afr J Infertil Assist Concept [Epub ahead of print] [cited 2021 Nov 28]. Available from: https://www.afrijiac.org/preprintarticle.asp?id=322999




  Abstract 1: Prevalence of infertility prior to conception amongst antenatal clinic attendees at the Rivers State University Teaching Hospital Top


Eli S, Kalio DGB1, Okagua K1, Green KI2, Kua P1, Omodu JO3

1Mother and Baby Care Global Foundation, Department of Obstetrics and Gynaecology and Rivers State University Teaching Hospital, 2Department of Obstetrics and Gynaecology, University of Port Harcourt Teaching Hospital, Port Harcourt, 3Department of Surgery, Rivers State University Teaching Hospital, Nigeria

Correspondence: Dr. Green KI,

2Department of Obstetrics and Gynaecology, University of Port Harcourt Teaching Hospital, Port Harcourt, Nigeria.

E-mail: [email protected]

Introduction: Infertility with its associated emotional and psychological consequences cuts across disciplines of gynaecology, urology and some instances psychiatry. Majority of researches on infertility involves the gynaecologist, however ante natal clinic attendees may also have their story to tell prior to pregnancy as regards infertility. The aim of this study is to determine the prevalence of infertility amongst antenatal clinic (ANC) attendees prior to conception at the Rivers State University Teaching Hospital (RSUTH).

Materials and Methods: It was a 6 weeks prospective study carried out at the department of obstetrics and gynaecology at the RSUTH. The information was retrieved with a self structured questionnaire and analyzed using SPSS version 25.

Results: Two hundred questionnaires were distributed and 184 retrieved. The mean age was 32.3 years. The modal parity was 1. The mean gestational age was 28 weeks. The prevalence of infertility prior to conception by ante natal clinic respondants was 14 (7.6 %). Prevalence of primary infertility was 4 (2.2%) while that of secondary infertility was 10 (5.4%). Cost 16 (8.7%) and accessibility 5 (2.7%) were the 2 most common reasons why these respondents did not opt for assisted reproduction prior to conception.

Conclusion: The study revealed that prevalence of infertility amongst antenatal clinic attendees at the RSUTH was 7.6% with secondary infertility commoner at 5.4%.

Keywords: Antenatal clinic attendees, infertility, prevalence, Rivers State University Teaching Hospital


  Abstract 2: Obesity and infertility: Weight management with Mayr-type detoxification and impact on fertility Top


Oladapo A Ashiru, Tinuke Adeyi, Adeola Bankole, Ebele Iloabachie Eunice Oribamise

Medical Art Center, and Mart-Life Detox Clinic, Lagos, Nigeria

Correspondence: Prof. Oladapo Ashiru,

Medical Art Center, Lagos, Nigeria.

E-mail: [email protected]

Introduction: Obese women experience impaired fertility in both natural and assisted conception cycles. Definitions of obesity can vary, but the most widely accepted definition is that of the World Health Organization (WHO), which uses the Body Mass Index (BMI) criterion. A person is obese if his/her BMI is more than or equal to 30Kg/M2.

Materials and Methods: 211 patients undergoing IVF treatment (from Jan 2016- February 2019) at the Medical ART Center (MART) Lagos, Nigeria were referred to MART-Life Detox clinic for a supervised Modern Mayr-type of detoxification before further Assisted Reproductive Technology (ART) treatment. The following criteria were used for referral: 1. History of Infertility. 2. Body mass index (BMI) greater than 27kg/m2. 3. History of prior failed ART attempt. Responders are those who fulfilled at least 2 of the criteria. 169 Patients (80%) experienced an improvement in their fertility outcomes and other laboratory parameters were assessed in subsequent cycles following detoxification.

Results: More than 93% of patients achieved significant weight reduction and an improvement in BMI. Uniform increase of about 62% in oocyte yield was noted. 42% of the patients tested positive for beta HCG following detoxification. Approximately 26% have carried live babies to term.

Conclusion: Mayr-type detoxification resulted in improved BMI, which had a correlation with increase in oocyte yield. A direct relationship was further found between increase in oocyte yield, fertilization and pregnancy rate following the detoxification program. The data suggests that supervised modern Mayr type of detoxification may positively impact fertility indices in infertile couples who undergo ART primarily by improving their BMI; however there is a need for randomized controlled trials in larger populations to further establish pattern of response.

Keywords: Modern Mayr-type detoxification, BMI, Assisted reproductive technology (ART), Fertility


  Abstract 3: Attitude of Nigerian gynaecologists towards gamete donation in Eastern Nigeria: A pilot study Top


Ezeome Ijeoma V, Nwankwo TO Umeh U

Department of Obstetrics and Gynaecology, UNTH, Enugu, Nigeria

Correspondence: Dr. Ezeome Ijeoma V,

Department of Obstetrics and Gynaecology, UNTH, Enugu, Nigeria.

E-mail: [email protected]

Introduction: Assisted Reproductive Technology (ART) as a treatment for infertility has come to stay in Nigeria. However, currently there is no national regulation of the practice. In the area of gamete donation, there is an international trend towards more information giving to donors/clients/child. We therefore sought to investigate the attitude towards gamete donation among Nigerian gynaecologists.

Materials and Methods: A descriptive study using self-administered questionnaires among attendees of Eastern sector meeting of Fellows of SOGON.

Results: 85% of our respondents were males, and 65% were less than 50years of age. 55% and 41% were of the view that there was no need for compensation for sperm and oocyte donation respectively. Majority did not agree that offsprings should receive information about donor in childhood nor as an adult.

Conclusion: The results indicate that Nigerian gynaecologist in Eastern Nigeria have a negative attitude to disclosure of genetic information to offsprings following gamete donation.

Keywords: Gamete donation, Nigeria, oocyte, sperm


  Abstract 4: Awareness of premarital fertility screening among women of child bearing age (21 to 45 years old) Top


Kayode-Lawal S

The Bridge Clinic, Ikeja, Lagos, Nigeria.

Correspondence: Mrs. Sekinat Kayode-lawal,

The Bridge Clinic, Ikeja, Lagos, Nigeria.

E-mail: [email protected]

Introduction: The desire to have one's own biological children can be strong and compelling; the effects of infertility for individuals or couples who are unable to conceive can be devastating. Infertility or its treatment can cause psychological stress, anxiety, and depression. Preventing infertility and the adverse consequences associated with its treatment are important concerns. Having a health screening before marriage provides a premarital preparation for couples. A clear need exists to identify public health priorities regarding infertility and its effect on health and also to create awareness on fertility testing before marriage. The study aims to assess the level of awareness of Fertility screening before Marriage among women of child bearing age, to determine if they are willing to know their fertility status before marriage and to create awareness on fertility testing before marriage.

Materials and Methods: This is a descriptive cross-sectional study. Questionnaires were distributed between May and June 2019 through WhatsApp to Nurses and Midwives within the child bearing age of 21 to 45 years old using a convenient sampling technique. 74 questionnaires were completely filled and analyzed.

Results: Majority (91.8%) of the respondents were knowledgeable about Infertility and most of the respondents (40%) heard about infertility from educational institutions. Majority of the respondents (86.49%) would want to know their fertility status and their partner's status before Marriage.

Conclusion: Most of the respondents heard about infertility from educational institutions, which is reflected on their level of awareness. The analysis also revealed that majority of the respondents are aware of infertility and are ready to ascertain their fertility status if provided the opportunity. The researcher however recommends that religious bodies should embrace the screening as part of routine testing for marriage, as this would further help create awareness about infertility and its management.

Keywords: Awareness, childbearing age, infertility, premarital fertility screening


  Abstract 5: Knowledge and attitude of ante natal clinic attendees towards assisted reproductive technology at a Tertiary Hospital in Rivers State, Nigeria Top


Eli S, Kalio DGB1, Fiebai P2, Okagua K1, Orazulike N2, Ikimalo J2

1Mother and Baby Care Global Foundation, Department of Obstetrics and Gynecology, Rivers State University Teaching Hospital, Port Harcourt, 2Department of Obstetrics and Gynaecology, University of Port Harcourt Teaching Hospital, Port Harcourt, Nigeria

Correspondence: Dr. Fiebai P,

Department of Obstetrics and Gynaecology, University of Port Harcourt Teaching Hospital, Port Harcourt, Nigeria.

E-mail: [email protected]

Introduction: Assisted reproductive technology (ART) has been accepted locally and globally as a conventional means for the treatment of infertility. Over 8million babies have been born as a result of invitro fertilization and other fertility treatment options. The aim of this study is ascertain the knowledge and attitude of antenatal clinic (ANC) attendees towards ART at the Rivers State University Teaching Hospital (RSUTH).

Materials and Methods: This was a cross-sectional study of ANC attendees at the RSUTH. A total of 200 questionnaires were distributed and 184 retrieved. Randomized sampling method was used. The data was analyzed using SPSS version 25.

Results: The mean age was 32.3 years and the modal parity was 1. For the level of education; tertiary 135 (73.4%), secondary 43 (23.4%), primary 1 (0.5%) and 5 (2.7%) of the respondents did not volunteer their educational level. One hundred and thirty three (72%) respondents had knowledge of ART. The most common ART method known by attendees was IVF 112 (60.9%), the least known was ICSI 2 (1.1%). 23 (12.5%) of respondents had ART in the past while 73 (39.7%) of attendees indicated the media as their most usual means of information.

Conclusion: The knowledge of ART (72%) amongst ANC attendees at the RSUTH was high. However, access is poor. 16% of respondents attributed this to high cost. Improving access through the public sector can drive down the cost of IVF in the country and many more people will be able to use these services.

Keywords: Antenatal clinic attendees, Assisted Reproductive Technology, attitude, knowledge


  Abstract 6: Successful treatment outcome of pre-implantation genetic testing for monogenic diseases (PGT-M): A case report from Nisa Hospital Abuja Top


Danga C, Tobi A, Chair M, Wada I

Fertility and Genetics Centre, Nisa Premier Hospital, Jabi District, Abuja, Nigeria

Correspondence: Dr. Christopher Danga,

Fertility and Genetics Centre, Nisa Premier Hospital,

Jabi District, Abuja, Nigeria.

E-mail: [email protected]

Introduction: Pre-implantation genetic testing for monogenic disease (PGT-M) is a useful technology for the treatment of couples with a high risk of transmitting genetic disorder to their children, such as sickle cell disease and autosomal disorders. We report the successful implantation and live birth outcome of a PGT-M treatment of a couple with the sickle cell disease (AS).

Case Description: A fertile couple, 27 years and 30 years old, female and male respectively were diagnosed with the sickle cell trait (AS carriers) and treated via intracytoplasmic sperm injection (ICSI). The initial treatment consisted of a short antagonist protocol of down regulation using 0.25mg of Cetrotide for 6 days, stimulated with 150IU of Recombinant Follicle Stimulating Hormone (FSH-r) for 11 days, Human Chorionic Gonadotropin (HCG) trigger with 0.5ml of Buserelin and oocyte retrieval was done 36 hours after HCG trigger. Of the 22 oocytes were retrieved, 20 matured metaphase II oocytes were injected with spouse's sperms leading to 18 oocytes fertilized and 14 embryos were biopsied. Diagnosis was done by SNP detection using minisequencing technique on Applied Biosystems 3500 genetic analyzer. Of the 12 analysed embryos, 5 were AA, 3 AS, 3 SS and 1 no diagnosis. The 2 AA were thawed and transferred. Urine pregnancy test and Beta HCG 10 days after FET yielded a positive result and clinical pregnancy was confirmed by Ultrasound after 3 weeks.

Discussion: The selection of genetically suitable embryos via PGT-M is fast improving the practice of assisted reproductive technology (ART) in Nigeria. Success rates of 64.4% have been reported for PGT-M in Canada (Butler et al, 2019). Factors associated with the success of this case report include ICSI protocol, embryo selection with high power PGT-M.

Conclusion: This probably, is the first case report of a successful live birth obtained from PGT-M in Nigeria. This case report evinces the availability and accessibility to advanced practice of ART with highly skilled manpower and quality management system in Nigeria.

Keywords: Embryo biopsy, intracytoplasmic sperm injection, monogenic disease, preimplantation genetic testing-M


  Abstract 7: Outcome of In Vitro Fertilization Treatment at Care Women's Clinic, Port Harcourt Top


Okandeji E, Aziken I, Azike P, Ulu N, Echehum E, Okolotu O, Okongwu C

Care Women's Clinic, Port Harcourt, Rivers, Nigeria

Correspondence: Ms. Elozino Okandeji,

Care Women's Clinic, Port Harcourt, Rivers, Nigeria.

E-mail: [email protected]

Introduction: The objective of this study is to determine the pregnancy outcome after in-vitro fertilization (IVF) and Intracytoplasmic sperm injection (ICSI) treatment at Care Women's Clinic from 2015.

Materials and Methods: This is a retrospective study of IVF/ ICSI cycles at the Clinic from January 2015 to December 2018. Couples who went through IVF treatment, and further had oocyte retrieval and embryo replacement procedures were included. A total of 945 cycles were analyzed. Outcomes were analyzed in terms of; implantation rates, clinical pregnancy rates and early pregnancy loss rates. Fertilization rates were also measured. Data analysis was done using Epi Info TM statistical software version 3.5.4.

Results: Data analysis of the 945 cycles; 477 cycles were conventional IVF and 468 were ICSI. The results were; Overall fertilization rates; 83.6%, implantation rates; 24.9%, clinical pregnancy rates; 49.4% and early pregnancy loss rates; 8.9%. Fertilization rates was significantly higher in the conventional IVF group with 81.9% as the ICSI group was 62.1%. However, implantation rates ( 28.9% vs 20.9%), clinical pregnancy rates (54.2% vs 44.5%) and early pregnancy loss rates (7.9% vs 10%) for conventional and ICSI were similar.

Conclusion: IVF treatment outcome at Care Women's Clinic, Port Harcourt was statistically comparable to global standard outcome.

Keywords: In vitro fertilization, intracytoplasmic sperm injection, Port Harcourt, pregnancy outcome


  Abstract 8: Preimplantation genetic testing using next generation sequencing in IVF: A clinical case report Top


Bamgbopa Kehinde T, Akinrinola O, John IR, Okorie LC, Olaide D, Akinsanya F, Emeka I

Olive Branch Fertility Centre, Lekki, Lagos, Nigeria

Correspondence: Dr. Bamgbopa Kehinde T,

Olive Branch Fertility Centre, Lekki, Lagos, Nigeria.

E-mail: [email protected]

Introduction: Though, an established adjunct to IVF worldwide, PGT in Nigeria is mostly dependent on oversea laboratory support. We report a successful completion of PGT-A/IVF treatment cycle using NGS, performed locally in Nigeria.

Clinical Case: A 30-year-old P1 with secondary infertility due to severe male factor; having had previous IVF success and determined to have only 2 children, couple insisted on PGT-A with gender selection. They were extensively counselled and consents for each stage discussed in an in-depth manner. She had controlled ovarian stimulation via antagonist protocol using Gonal F. At egg retrieval, 36 hours post 10,000 IU of Pregnyl, 21 oocytes were collected. Nine embryos reached the blastocyst stage on day 5 Post-ICSI. Trophectoderm biopsy was performed using a multipulse contact laser (Lykos, Hamilton Thorne, UK). An average of 2 trophectoderm cells removed per blastocyst were sent for PGT-A, performed by NGS using the Ion Torrent method. The outcomes were 2 (XY) male blastocysts, 3 (XX) female blastocysts, and 4 inconclusive blastocysts. The aneuploidy status of all the blastocysts biopsied was documented and thoroughly discussed with the couple. Thereafter, the 2 (XY) male blastocysts were transferred (ET) 15 hours post-embryo biopsy. Serum pregnancy test was positive 14 days post-ET and Ultrasonography confirmed an intrauterine pregnancy 2 weeks later. Resultant pregnancy is ongoing at EGA of 30 weeks at the time of this report.

Conclusion: This is the first case demonstrating the successful clinical use of NGS PGT-A to achieve ET on same day as biopsy and resulting in an ongoing pregnancy in Nigeria.

Keywords: In vitro fertilization, next generation sequencing, preimplantation genetic testing-A


  Abstract 9: Blastulation rates: Is it higher by traditional in vitro fertilization or ICSI insemination? Top


Danga C, Eneojo AJ, Nwanganga I, Amatu P, Ekeruche I, Wada I

Fertility and Genetics Centre, Nisa Premier Hospital, Jabi District, Abuja, Nigeria

Correspondence: Ms. Ifeoma Nwanganga,

Fertility and Genetics Centre, Nisa Premier Hospital,

Jabi District, Abuja, Nigeria.

E-mail: [email protected]

Introduction: Insemination by ICSI is receiving growing attention over traditional IVF; especially performed to improve possibility of fertilization where potential challenges such as poor semen quality and previous fertilization failures exist. In this study, we hypothesised that insemination by ICSI provides higher blastulation rates than traditional IVF.

Materials and Methods: This is a retrospective study of 360 cycles collected between Jan 2017 and Jul 2019 at Nisa Hospital Abuja. Only IVF or ICSI inseminations were included, split cycles were excluded. The Gardner's blastocyst grading scale was used and 3BB or better grade were considered as good quality blastocyst. ICSI vs. IVF blastulation rates were compared using Analyse-it® v4.7 method validation statistical software.

Results: The proportion of IVF (68%) insemination was significantly higher than ICSI (32%), (Binomial test). Wilcoxon-Mann-Whitney tests shows no statistically significant difference between the two insemination methods in terms of age of women, cleaved and good quality blastocyst proportions, but fertilization rate was significantly higher in ICSI than IVF as expected.

Conclusion: Embryo cleavage is critical to blastulation rates. But insemination by ICSI does not provide higher blastulation rates than traditional IVF.

Keywords: Blastulation rates, in vitro fertilization, ICSI insemination, fertilization


  Abstract 10: Serum progesterone (P4) levels association with pregnancy rates in in vitro fertilization/embryo transfer cycles Top


Odeku T, Monday NS, Ameh N, Wada I

Fertility and Genetics Centre, Nisa Premier Hospital, Jabi District, Abuja, Nigeria

Correspondence: Dr. Thompson Odeku,

Fertility and Genetics Centre, Nisa Premier Hospital,

Jabi District, Abuja, Nigeria

E-mail: [email protected]

Introduction: There is still controversy over serum progesterone (P4) threshold levels and its association with successful implantation and improved pregnancy rates in in vitro fertilization/ embryo transfer (IVF/ET). This study presents an assessment of the relationship between P4 levels and pregnancy outcomes.

Materials and Methods: A prospective analysis of 120 consecutive patients data undergoing stimulation for IVF/ET in which serial serum P4 levels were measured between Jan 2016 and Dec. 2018. Three groups of P4 levels were assigned; GP 1 (<1.27 nmol/L), Gp 2 (1.27 nmol/L to <2.86 nmol/L) and Gp 3 (≥2.86 nmol/L) and were correlated with day of hCG administration.

Results: Clinical pregnancies were recorded in 22 of 69 (32%) patients in GP 1 compared to 11 of 37 patients (30%) in GP 2. No pregnancy was recorded in GP 3 14 (0%) patients (Figure 1). There was no statistically significant difference in pregnancies Chi-squared-test (p >.05) and P4 levels t-test (p >.05) between GP 1 and GP 2. These were in contrast to previous study1.

Conclusion: These findings further substantiate the position that increases in serum P4 levels >1.27 nmol/L are associated with reduced pregnancy rates in IVF/ET cycles.

Keywords: Serum progesterone, pregnancy rates, in vitro fertilization, embryo transfer






 

 
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Abstract 1: Prev...
Abstract 2: Obes...
Abstract 3: Atti...
Abstract 4: Awar...
Abstract 5: Know...
Abstract 6: Succ...
Abstract 7: Outc...
Abstract 8: Prei...
Abstract 9: Blas...
Abstract 10: Ser...

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